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primescript rtreagent kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript rtreagent kit
    Primescript Rtreagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rtreagent+kit/PrimeScript+RT+Reagent+Kit/pm41720949-227-28-31
    Average 99 stars, based on 88323 article reviews
    primescript rtreagent kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Robinin attenuates cardiac hypertrophy in pulmonary heart disease by modulating SIRT1/NF-κB signaling and inhibiting oxidative stress and the NLRP3 inflammasome.
    Article Snippet: Total RNA was isolated using TRIzol reagent (Invitrogen, USA) and quantified spectrophotometrically (NanoDrop 2000, Thermo Fisher Scientific, USA). .. Reverse transcription was performedwith the PrimeScript RTReagent Kit (Takara, Japan) following the manufacturer’s protocol. .. Real-time PCR amplification was conducted on the ABI 7300 system (Thermo Fisher Scientific, USA) using SYBR Premix Ex Taq II (Takara, Japan).

    Article Title: African strains of Zika virus resist ISG-mediated restriction.
    Article Snippet: RNA concentration and purity were evaluated by spectrophotometry (NanoDrop 2000c, Thermo Fisher Scientific). .. A maximum of 500 ng of RNA were reverse transcribed with both oligo dT and random primers using a PrimeScript RTReagent Kit (Perfect Real Time, Takara Bio Inc.) in a 10 μL reaction. .. Real-time PCR reactions were performed in triplicate using Master Mix PowerUp SYBR Green (Thermo Fisher Scientific) on an Applied Biosystems ViiA 7 Real-Time PCR System (Thermo Fisher Scientific).

    Article Title: ARRB2 promotes cervical cancer progression via stabilizing CDC25A mRNA through m6A-IGF2BP1-dependent manner.
    Article Snippet: Sections were counter-stained with hematoxylin (Beyotime) and imaged. qRT-PCR RNAwas extracted from tumor and para-carcinoma tissues and cells using TRIzol reagent (Thermo Fisher Scientific). .. RNA quality was examined by electrophoresis, and then reverse transcription was performed using PrimeScript RTReagent Kit (Takara, Kusatsu, Shiga, Japan). .. The expression of ARRB2, CDC25A and IGF2BP1 was determined through real-time quantitative PCRwith SYBR green (Thermo Fisher Scientific) and normalized to GAPDH.

    Article Title: FBXO31-mediated ubiquitination of OGT maintains O-GlcNAcylation homeostasis to restrain endometrial malignancy.
    Article Snippet: RNA extraction was performed using TRIzol (87804, Life Technologies) according to the manufacturer’s protocol for all samples, including cells, organoids, and primary tissues. .. The extracted RNAwas then reverse transcribed to cDNAusing the PrimeScript RTReagent Kit (RR037A, Takara). .. The cDNA was used as a template for qPCR, which was performed using the SYBR Green qPCR Master Mix (QST-100, SolomonBio) on the QuantStudio 3 Real-Time PCR system (Applied Biosystems).

    Article Title: African strains of Zika virus resist ISG-mediated restriction
    Article Snippet: RNA concentration and purity were evaluated by spectrophotometry (NanoDrop 2000c, Thermo Fisher Scientific). .. A maximum of 500 ng of RNA were reverse transcribed with both oligo dT and random primers using a PrimeScript RTReagent Kit (Perfect Real Time, Takara Bio Inc.) in a 10 μL reaction. .. Real-time PCR reactions were performed in triplicate using Master Mix PowerUp SYBR Green (Thermo Fisher Scientific) on an Applied Biosystems ViiA 7 Real-Time PCR System (Thermo Fisher Scientific).

    Electrophoresis:

    Article Title: ARRB2 promotes cervical cancer progression via stabilizing CDC25A mRNA through m6A-IGF2BP1-dependent manner.
    Article Snippet: Sections were counter-stained with hematoxylin (Beyotime) and imaged. qRT-PCR RNAwas extracted from tumor and para-carcinoma tissues and cells using TRIzol reagent (Thermo Fisher Scientific). .. RNA quality was examined by electrophoresis, and then reverse transcription was performed using PrimeScript RTReagent Kit (Takara, Kusatsu, Shiga, Japan). .. The expression of ARRB2, CDC25A and IGF2BP1 was determined through real-time quantitative PCRwith SYBR green (Thermo Fisher Scientific) and normalized to GAPDH.

    Isolation:

    Article Title: Adipose tissue-derived MFG-E8 promotes hepatic inflammation and fibrosis through macrophage activation in a mouse MASH model.
    Article Snippet: .. Total RNA was isolated from tissues or cultured cells with the use of the RNAiso Plus reagent (Takara) and was subjected to RT with the use of a PrimeScript RTReagent Kit (Takara). .. The resulting cDNAwas subjected to qPCR analysis with SYBR Green Master Mix (Applied Biosystems) and specific primers (Supplementary Table 1) in a StepOnePlus instrument (AppliedBiosystems).

    Cell Culture:

    Article Title: Adipose tissue-derived MFG-E8 promotes hepatic inflammation and fibrosis through macrophage activation in a mouse MASH model.
    Article Snippet: .. Total RNA was isolated from tissues or cultured cells with the use of the RNAiso Plus reagent (Takara) and was subjected to RT with the use of a PrimeScript RTReagent Kit (Takara). .. The resulting cDNAwas subjected to qPCR analysis with SYBR Green Master Mix (Applied Biosystems) and specific primers (Supplementary Table 1) in a StepOnePlus instrument (AppliedBiosystems).

    Synthesized:

    Article Title: Self-reinforced piezoelectric chip for scaffold-free repair of critical-sized bone defects.
    Article Snippet: The RNA concentration was determined by a spectrophotometer (Nanodrop one, ThermoFisher Scientific). .. Complementary DNA was synthesized using PrimeScript RTReagent kit (RR037A, Takara, Japan). ..

    other:

    Article Title: Targeting the AURKB-MAD2L2 Axis Disrupts the DNA Damage Response and Glycolysis to Inhibit Colorectal Cancer Progression
    Article Snippet: Total RNA was extracted from CRC cells using TRIzol reagent (9109, Takara, Dalian, China), and complementary DNA (cDNA) was synthesized using the PrimeScriptTMRTReagent Kit (RR047A, Takara, Dalian, China).



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